Please use this identifier to cite or link to this item:
|Title:||Distribution of calcium-independent phospholipase A2 (iPLA 2) in monkey brain|
|Citation:||Ong, W.-Y., Yeo, J.-F., Ling, S.-F., Farooqui, A.A. (2006). Distribution of calcium-independent phospholipase A2 (iPLA 2) in monkey brain. Journal of Neurocytology 34 (6) : 447-458. ScholarBank@NUS Repository.|
|Abstract:||The present study was carried out to elucidate the distribution of calcium-independent phospholipase A2 (iPLA2) in the normal monkey brain. iPLA2 immunoreactivity was observed in structures derived from the telencephalon, including the cerebral neocortex, amygdala, hippocampus, caudate nucleus, putamen, and nucleus accumbens, whereas structures derived from the diencephalon, including the thalamus, hypothalamus and globus pallidus were lightly labeled. The midbrain, vestibular, trigeminal and inferior olivary nuclei, and the cerebellar cortex were densely labeled. Immunoreactivity was observed on the nuclear envelope of neurons, and dendrites and axon terminals at electron microscopy. Western blot analysis showed higher levels of iPLA2 protein in the cytosolic, than the nuclear fraction, but little or no protein in the membrane fraction. Similarly, subcellular fractionation studies of iPLA2 activity in rat brain cortical cell cultures showed greater enzymatic activity in the cytosolic, than the nuclear fraction, and the least activity in non-nuclear membranes. The association of iPLA2 with the nuclear envelope suggests a role of the enzyme in nuclear signaling, such as during neuronal proliferation and differentiation or death. In addition, the localization of iPLA2 in dendrites and axon terminals suggests a role of the enzyme in neuronal signaling. © Springer Science + Business Media, LLC 2006.|
|Source Title:||Journal of Neurocytology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 28, 2018
WEB OF SCIENCETM
checked on Apr 24, 2018
checked on Dec 15, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.