Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.tec.2009.0360
Title: Autologous feeder cells from embryoid body outgrowth support the long-term growth of human embryonic stem cells more effectively than those from direct differentiation
Authors: Fu, X.
Toh, W.S. 
Liu, H. 
Lu, K.
Li, M.
Hande, M.P.
Cao, T. 
Issue Date: 2010
Source: Fu, X., Toh, W.S., Liu, H., Lu, K., Li, M., Hande, M.P., Cao, T. (2010). Autologous feeder cells from embryoid body outgrowth support the long-term growth of human embryonic stem cells more effectively than those from direct differentiation. Tissue Engineering - Part C: Methods 16 (4) : 719-733. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tec.2009.0360
Abstract: Autologous feeder cells have been developed by various methods to minimize the presence of xenogenic entities in human embryonic stem cell (hESC) cultures. However, there was no systematic comparison of supportive effects of the feeder cells on hESC growth, nor comparison to the supportive effects of various feeder-free culture systems and standard mouse feeder cells. In this study, we aimed to compare the supportive abilities of autologous feeders derived either directly from H9 hESCs (H9 dF) or from outgrowth of embryoid body predifferentiated in suspension from H9 hESCs (H9 ebF). Mouse feeder system and matrigel-mTeSR1 feeder-free system were used as controls. H9 ebF was found to secrete more basic fibroblast growth factor in the conditioned medium than H9 dF did. The undifferentiated state of H9 hESCs was sustained more stably on H9 ebF than on H9 dF, and the differentiation potential of H9 hESCs on H9 ebF was higher than on H9 dF. We concluded that H9 ebF was an optimal autologous feeder to maintain the long-term undifferentiated state of hESCs in our current culture system. This study helps to standardize the autologous culture of hESCs. It also suggests a more definite direction for future development of xeno-free culture system for hESCs. © 2010 Mary Ann Liebert, Inc.
Source Title: Tissue Engineering - Part C: Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/46577
ISSN: 19373384
DOI: 10.1089/ten.tec.2009.0360
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

22
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

14
checked on Dec 13, 2017

Page view(s)

59
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.