Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/43730
Title: The Development of Acidic Protein Aptamers using Capillary Electrophoresis Methods and their use in Surface Plasmon Resonance
Authors: JON ASHLEY
Keywords: Aptamers, SELEX, Biosensors, protein, Surface plasmon Resonance
Issue Date: 28-Mar-2013
Source: JON ASHLEY (2013-03-28). The Development of Acidic Protein Aptamers using Capillary Electrophoresis Methods and their use in Surface Plasmon Resonance. ScholarBank@NUS Repository.
Abstract: Aptamers are ssDNA and RNA that bind to a number of biomolecules including proteins, cells and small molecules as an alternative to antibodies. Capillary based selections known as CE-SELEX and Non-SELEX has been proposed as alternative mode for selecting aptamers. In the first parts of this thesis, these techniques were used to select aptamers for bovine catalase, human leptin and human haemoglobin and some of the limitations associated with these techniques were improved upon. An alternative CE based method for the selection of aptamers was then proposed called Hybridised-SELEX. This technique allows for the number of sequences screened to be increased while allowing the selection to be carried out in fewer rounds. This technique was used to select cholesterol esterase aptamers. In the last part of the thesis, the bovine catalase aptamer was used to develop an aptamer based SPR biosensor for the screening of mastitis disease in milk.
URI: http://scholarbank.nus.edu.sg/handle/10635/43730
Appears in Collections:Ph.D Theses (Open)

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