Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/43595
Title: TARGETING INOSITOL LIPIDS THROUGH METABOLIC LABELLING WITH INOSITOL ANALOGS
Authors: SHAREEF MOHIDEEN ISMAIL
Keywords: Azido-inositol analogs, Inositol lipids, click chemistry, metabolic labelling, yeast, mass spectrometry
Issue Date: 27-Nov-2012
Source: SHAREEF MOHIDEEN ISMAIL (2012-11-27). TARGETING INOSITOL LIPIDS THROUGH METABOLIC LABELLING WITH INOSITOL ANALOGS. ScholarBank@NUS Repository.
Abstract: Myo-inositol is biosynthesized from glucose-6-phosphate or transported from culture media for inositol lipid biosynthesis. Phosphatidylinositol is the parent inositol lipid from which phosphoinositides, GPI anchors and other lipids are generated ¿ a pathway that is highly conserved amongst eukaryotes. We target inositol lipids using variations in substrate preferences of enzymes in the inositol lipid biosynthesis pathway. Azide-modified myo-inositol was synthesized with the azide moiety allowing for specific labelling with alkyne-modified reporter molecules through Azide-alkyne Huisgen cycloaddition (click chemistry). Saccharomyces cerevisiae was selected as the model organism to study the effects of inositol analogs on growth and cellular environment. Incorporation of azido-inositol into lipids was confirmed and modified lipids were tagged specifically. Changes in lipid homeostasis were observed in the presence of azido-inositol when compared with myo-inositol supplementation, although gene elements were regulated in a similar manner. Azido-inositol shows potential as a tool for studying the localization and metabolism of inositol lipids.
URI: http://scholarbank.nus.edu.sg/handle/10635/43595
Appears in Collections:Ph.D Theses (Open)

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