Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/43431
Title: Translatomics Study of Chinese Hamster Ovary Cell Cultures
Authors: COURTES FRANCK CEDRIC
Keywords: Translatome, CHO cells, snoRNA U19, mTOR pathway, translation
Issue Date: 10-Jan-2013
Source: COURTES FRANCK CEDRIC (2013-01-10). Translatomics Study of Chinese Hamster Ovary Cell Cultures. ScholarBank@NUS Repository.
Abstract: We report the first investigation of translational efficiency on a global scale, also known as translatome, of a CHO DG44 cell line producing monoclonal antibodies. The translatome data was generated via combined use of high-resolution and streamlined polysome profiling technology and microarrays probing for more than 13K annotated CHO-specific genes. The distribution of ribosome loading during the exponential growth phase revealed the translational activity corresponding to the maximal growth rate, thus allowing us to identify stably and highly translated genes as potential key players for cellular growth. Moreover, correlation analysis between transcriptome and translatome data sets suggested the implication of translational control mechanisms such as the mTOR pathway. Thus, the current translatome analysis platform enables researchers of the bioprocessing field to prioritize high-potential candidate genes and to devise optimal strategies for cell engineering towards improving culture performances.
URI: http://scholarbank.nus.edu.sg/handle/10635/43431
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
CourtesFC.pdf8.55 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

213
checked on Dec 18, 2017

Download(s)

319
checked on Dec 18, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.