Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/43415
Title: Modulation of Nuclear Factor -?B signaling attenuates allergic airway inflammation
Authors: GOH YIQIAN FERA
Keywords: Lung epithelium , Ovalbumin, asthma, mucus hypersecretion, siRNA, respiratory
Issue Date: 2-Jan-2013
Source: GOH YIQIAN FERA (2013-01-02). Modulation of Nuclear Factor -?B signaling attenuates allergic airway inflammation. ScholarBank@NUS Repository.
Abstract: Asthma is an airway inflammatory disease that is associated with persistent activation of NF-kappaB signaling pathway. NF-kappaB is a master pro-inflammatory transcription factor. Using mouse asthma model and/or TNF-alpha stimulated lung cell lines, we demonstrated the potential anti-inflammatory action of NF-kappaB signaling pathway interfering molecules: (1) Rip-2 siRNA; (2) RPS-3 siRNA; and (3) bioactive flavonol fisetin. In mouse asthma model, Rip-2 siRNA and fisetin blocked OVA-induced (a)inflammatory cell infiltration and mucus hypersecretion in the airways; (b)mRNA expression of adhesion molecules and inflammatory mediators in lung tissues; and (c) OVA-specific IgE level in serum. Furthermore, Rip-2 siRNA and fisetin-treated mice produced significantly less airway hyperresponsiveness induced by methacholine. On the other hand, in TNF-a-stimulated cell lines, RPS-3 siRNA suppressed TNF-alpha-induced MUC5AC expression and IL-8 production. Taken together, our results suggest that Rip-2 siRNA, RPS-3 siRNA, and fisetin may have therapeutic potential for treatment of allergic airway inflammation.
URI: http://scholarbank.nus.edu.sg/handle/10635/43415
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