Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38843
Title: STUDY OF DENGUE INFECTION IN A HUMANIZED MOUSE MODEL
Authors: AISHWARYA SRIDHARAN
Keywords: dengue virus, humanized mice, pathogenesis, platelets, thrombocytopenia, host tropism
Issue Date: 14-Feb-2013
Source: AISHWARYA SRIDHARAN (2013-02-14). STUDY OF DENGUE INFECTION IN A HUMANIZED MOUSE MODEL. ScholarBank@NUS Repository.
Abstract: A road block in the study of dengue disease is the lack of a small animal model particularly for some of its clinical symptoms. By adoptive transfer of human CD34+ fetal liver cells into immunodeficient mice, we have constructed humanized mice with significant levels of human platelets, monocytes/macrophages and hepatocytes. Infection of these mice with dengue virus produces systemic infection along with characteristic clinical symptoms such as transient leukopenia, liver damage and thrombocytopenia. This model was used to investigate the mechanism underlying dengue-induced thrombocytopenia in a human cell context. In addition this model was also used to study the differences in replicating ability of the virus that could result in a change in its host tropism. Though there are drawbacks, this model can be used in a variety of ways to understand the complex host-viral interactions. Overcoming the limitations could make the model even more useful for studying dengue disease.
URI: http://scholarbank.nus.edu.sg/handle/10635/38843
Appears in Collections:Ph.D Theses (Open)

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