Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38821
Title: MOLECULAR ANALYSIS OF THE ROLE OF YEAST MITOCHONDRIAL PROTEIN QCR7P IN AGROBACTERIUM-YEAST DNA TRANSFER
Authors: YANG QINGHUA
Keywords: Agrobacterium, T-DNA, QCR7, Mitochondrion, VirE2, visualization
Issue Date: 23-Jan-2013
Source: YANG QINGHUA (2013-01-23). MOLECULAR ANALYSIS OF THE ROLE OF YEAST MITOCHONDRIAL PROTEIN QCR7P IN AGROBACTERIUM-YEAST DNA TRANSFER. ScholarBank@NUS Repository.
Abstract: Agrobacterium tumefaciens has a unique capability to horizontally transfer DNA into host plants as well as some non-natural recipients. The virulence machinery of A. tumefaciens facilitating the DNA transfer has been intensively investigated, while the relevant host factors are less understood. In this study, Saccharomyces cerevisiae is employed to investigate how host factors are involved in the process. Here I show that a mitochondrial protein Qcr7p was involved in the transfer process in a negative manner. This was due to an increase in the competency of the yeast to receive both T-DNA and virulence effectors. To detect the virulence effectors in recipients, a novel approach was established to visualize bacterium-delivered VirE2 molecules. This is the first time that bacterium-originated VirE2 and its movement were visualized inside recipient cells. This technique enables molecular dissection of the intracellular trafficking pathway of VirE2 and T-complex delivered by Agrobacterium biosyringe.
URI: http://scholarbank.nus.edu.sg/handle/10635/38821
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Role of Yeast Qcr7p in Agrobacterium-mediated transformation.pdf3.4 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

171
checked on Dec 11, 2017

Download(s)

34
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.