Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38811
Title: Simulation Of Auto-Inhibition Effect In Mev Ntail With Its Binding Partner XD
Authors: WENWEI XIANG
Keywords: MeV, Ntail, XD, Intrinsically Disordered Protein (IDP), Auto-inhibition Effect, Drug Design
Issue Date: 21-Mar-2013
Citation: WENWEI XIANG (2013-03-21). Simulation Of Auto-Inhibition Effect In Mev Ntail With Its Binding Partner XD. ScholarBank@NUS Repository.
Abstract: An in-silico simulation of large conformational ensembles of the intrinsically disordered portion of MeV Ntail was used to examine the conformational space and collisions involved in binding to the polymerase P protein. One million conformers of Ntail were generated with populations of binding motif constrained to helix fractions derived from published NMR experiments. Another 0.5 million structures were generated with predicted fractions of secondary structure using the GOR method. After dual threshold screening, results show Ntail auto-inhibitory effect exists and is helix length dependent. An asymmetric effect between the flanking region at the C-terminus of the helix and the N-terminus is first verified. The GOR method ensemble show similar results and can be used to predict these properties of Ntail without NMR data. The study sheds light on the structural basis of auto-inhibition, structural binding asymmetry when Ntail interacts with XD and to the therapeutic drug design against MeV.
URI: http://scholarbank.nus.edu.sg/handle/10635/38811
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
XiangWW.pdf4.27 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

158
checked on Oct 13, 2018

Download(s)

197
checked on Oct 13, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.