Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38808
Title: Microencapsulation of Clostridium acetobutylicum Cells and Utilisation of Samanea saman Leaves for the Production of Biobutanol
Authors: SWETA RATHORE
Keywords: Microencapsulation, biobutanol, hydrolysis, fermentation, optimisation, spores
Issue Date: 23-Jan-2013
Source: SWETA RATHORE (2013-01-23). Microencapsulation of Clostridium acetobutylicum Cells and Utilisation of Samanea saman Leaves for the Production of Biobutanol. ScholarBank@NUS Repository.
Abstract: Economical butanol production using Clostridium acetobutylicum has faced difficulties due to poor solvent tolerance of the bacteria and high cost of fermentation substrates. In this study, clostridial cell encapsulation in gellan gum microspheres was investigated as a strategy to protect the cells from harsh conditions encountered during fermentation. Compared to free cells, the encapsulated cells could be easily recovered and reused with high fermentation efficiency, albeit with significant cell leakage. The microspheres served as cell nurseries to provide free cells for fermentation. Furthermore, the potential of Samanea saman leaf litter as a source of fermentable sugars for butanol production was explored. The influence of milling, hydrothermal treatment and enzyme/acid hydrolysis of the leaf litter on sugar yields and suitability of various detoxification methods for the acid hydrolysate were investigated. From the results, the leaf litter was successfully exploited as a fermentation substrate for biobutanol production by encapsulated clostridial cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/38808
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