Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38755
Title: INFLAMIN FROM AIPYSURUS EYDOUXII: A NOVEL TOXIN INDUCING INFLAMMATION
Authors: BHASKAR BARNWAL
Keywords: Prostacyclin, Edema, Inflammation, Cyclooxygenase, Cytotoxic, Phosphorylation
Issue Date: 23-Aug-2012
Source: BHASKAR BARNWAL (2012-08-23). INFLAMIN FROM AIPYSURUS EYDOUXII: A NOVEL TOXIN INDUCING INFLAMMATION. ScholarBank@NUS Repository.
Abstract: We expressed a cysteine-rich, secretory protein containing 94 amino acid residues that induced inflammation and writhing in animals, and was named as inflamin. It induced two waves of prostanoids production. The first wave peaked at 10 min and 6-keto PGF1a was the major product. The second wave, specifically of 6-keto PGF1a and PGE2, started after 2 h. In RAW264.7 cells, COX-1 activity showed a transient increase at 10 min, but its expression was unaffected. COX-2 was induced after 3 h and is responsible for the second wave. Using specific inhibitors, we showed that cPLA2 plays a key role in arachidonate release. The cPLA2 activity showed a transient increase of 62% at 10 min due to its phosphorylation at Ser505. Thus, inflamin - the first member of a new family of snake venom proteins, leads to an increase in the cPLA2 and COX-1 activity resulting in inflammation and pain.
URI: http://scholarbank.nus.edu.sg/handle/10635/38755
Appears in Collections:Ph.D Theses (Open)

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