Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38703
Title: Functional Study Of MicroRNA-125b In Vertebrate Development
Authors: LE THI NGUYET MINH
Keywords: microRNA, development, biology, system biology, vertebrate, miR-125b
Issue Date: 30-Jul-2009
Source: LE THI NGUYET MINH (2009-07-30). Functional Study Of MicroRNA-125b In Vertebrate Development. ScholarBank@NUS Repository.
Abstract: microRNAs are a class of small non-coding RNAs that regulate gene expression at the<br>post-transcriptional level. This thesis aims to reveal the new functions of miR-125b, a<br>brain-enriched microRNA in vertebrate development. First, we demonstrated the<br>important role of miR-125b in differentiation of two human neural cell lines. We also<br>found that a subset of miR-125b-repressed targets antagonize neuronal genes in<br>several neurogenic pathways. Second, we demonstrated that miR-125b is<br>indispensable for zebrafish embryogenesis, particularly for the survival of neural<br>cells. We identified p53, a key tumor suppressor, as a bona fide target of miR-125b in<br>both zebrafish and humans. miR-125b directly represses p53 and multiple genes in the<br>p53 network while p53 in turn suppressed the expression of miR-125b. Together, our<br>study provides a global view of miR-125b function in modulating gene expression to<br>maintain the homeostasis of cell survival, death and differentiation during<br>development.
URI: http://scholarbank.nus.edu.sg/handle/10635/38703
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