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Title: | Functional Study Of MicroRNA-125b In Vertebrate Development | Authors: | LE THI NGUYET MINH | Keywords: | microRNA, development, biology, system biology, vertebrate, miR-125b | Issue Date: | 30-Jul-2009 | Citation: | LE THI NGUYET MINH (2009-07-30). Functional Study Of MicroRNA-125b In Vertebrate Development. ScholarBank@NUS Repository. | Abstract: | microRNAs are a class of small non-coding RNAs that regulate gene expression at the post-transcriptional level. This thesis aims to reveal the new functions of miR-125b, a brain-enriched microRNA in vertebrate development. First, we demonstrated the important role of miR-125b in differentiation of two human neural cell lines. We also found that a subset of miR-125b-repressed targets antagonize neuronal genes in several neurogenic pathways. Second, we demonstrated that miR-125b is indispensable for zebrafish embryogenesis, particularly for the survival of neural cells. We identified p53, a key tumor suppressor, as a bona fide target of miR-125b in both zebrafish and humans. miR-125b directly represses p53 and multiple genes in the p53 network while p53 in turn suppressed the expression of miR-125b. Together, our study provides a global view of miR-125b function in modulating gene expression to maintain the homeostasis of cell survival, death and differentiation during development. |
URI: | http://scholarbank.nus.edu.sg/handle/10635/38703 |
Appears in Collections: | Ph.D Theses (Open) |
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LeTNM-part1.pdf | 10.54 MB | Adobe PDF | OPEN | None | View/Download | |
LeTNM-part2.pdf | 9.69 MB | Adobe PDF | OPEN | None | View/Download |
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