Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/38383
Title: TOWARDS DETERMINATION OF NATURAL VARIATION OF BLOOD LIPIDS IN THE SINGAPOREAN POPULATION
Authors: HUSNA BEGUM
Keywords: lipidomics, technical variation, biological variation, mass spectrometry, healthy population
Issue Date: 24-Jan-2013
Source: HUSNA BEGUM (2013-01-24). TOWARDS DETERMINATION OF NATURAL VARIATION OF BLOOD LIPIDS IN THE SINGAPOREAN POPULATION. ScholarBank@NUS Repository.
Abstract: Many unanswered questions remain on the impact of genome natural variation in healthy populations. The basis of the biological variation cannot be completely explained by genomics and proteomics. Lipidomics offers an avenue to complement these traditional technologies, studying lipid profiles across various conditions, and the recent advances in mass spectrometry complemented existing platforms in providing rapid and sensitive methods for lipids profiling. Lipids have been found to be important in human health as their dysregulation has been reported in pathological conditions. It is, however, imperative to understand how lipids vary in normal physiological settings. As such, this study aims to explore the range of biological variation in human plasma of various lipids including glycerophospholipids, sphingolipids and sterol derivatives, in 360 healthy fasting Singaporeans. Targeted mass spectrometry using multiple reaction monitoring (MRM) was used to quantify over 100 individual lipid species. In a first step, variation that is introduced by sample pre-processing and instrumentation, i.e. technical variation, has been carefully considered and the importance of using quality control samples in study design was recognized. Issues involving the analysis of multiple batches by mass spectrometry and batch effect corrections were addressed prior to considering any biological variation. In this study, we identified ethnicity dependent differences across lipid species, in particular significantly higher levels of PS and pPE and pPC in Chinese when compared to Indian and Malay populations. Ultimately, this work aims to quantify the biological variation of lipids in the healthy Singaporean population across three ethnicities (Chinese, Malay and Indian) and to understand the biological meaning of the underlying mechanisms.
URI: http://scholarbank.nus.edu.sg/handle/10635/38383
Appears in Collections:Ph.D Theses (Open)

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