Please use this identifier to cite or link to this item:
https://doi.org/10.1016/S0891-5849(01)00647-5
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dc.title | Increased formation of S-nitrothiols and nitrotyrosine in cirrhotic rats during endotoxemia | |
dc.contributor.author | Ottesen, L.H. | |
dc.contributor.author | Harry, D. | |
dc.contributor.author | Frost, M. | |
dc.contributor.author | Davies, S. | |
dc.contributor.author | Khan, K. | |
dc.contributor.author | Halliwell, B. | |
dc.contributor.author | Moore, K. | |
dc.date.accessioned | 2013-06-05T09:49:05Z | |
dc.date.available | 2013-06-05T09:49:05Z | |
dc.date.issued | 2001 | |
dc.identifier.citation | Ottesen, L.H., Harry, D., Frost, M., Davies, S., Khan, K., Halliwell, B., Moore, K. (2001). Increased formation of S-nitrothiols and nitrotyrosine in cirrhotic rats during endotoxemia. Free Radical Biology and Medicine 31 (6) : 790-798. ScholarBank@NUS Repository. https://doi.org/10.1016/S0891-5849(01)00647-5 | |
dc.identifier.issn | 08915849 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/38249 | |
dc.description.abstract | Plasma S-nitrosothiols are believed to function as a circulating form of nitric oxide that affects both vascular function and platelet aggregation. However, the formation of circulating S-nitrosothiols in relation to acute and chronic disease is largely unknown. Plasma S-nitrosothiols were measured by chemiluminescence in rats with biliary cirrhosis or controls, and the effect of lipopolysaccharide (LPS) on their formation was determined. Plasma S-nitrosothiols were increased in rats with cirrhosis (206 ± 59 nM) compared to controls (51 ± 6 nM, p < .001). Two hours following injection of LPS (0.5 mg/kg) plasma S-nitrosothiols increased to 108 ± 23 nM in controls (p < .01) and to 1335 ± 423 nM in cirrhotic rats (p < .001). The plasma clearance and half-life of S-nitrosoalbumin, the predominant circulating S-nitrosothiol, were similar in control and cirrhotic rats, confirming that the increased plasma concentrations were due to increased synthesis. Because reactive nitrogen species, such as peroxynitrite, may cause the formation of S-nitrosothiols in vivo, we determined the levels of nitrotyrosine by gas chromatography/mass spectrometry as an index for these nitrating and nitrosating radicals. Hepatic nitrotyrosine levels were increased at 7.0 ± 1.2 ng/mg in cirrhotic rats compared to controls (2.0 ± 0.2 ng/mg, p < .01). Hepatic nitrotyrosine levels increased by 2.3-fold and 1.5-fold in control and cirrhotic rats, respectively, at 2 h following injection of LPS (p < .01). Strong positive staining for nitrotyrosine was shown by immunohistochemistry in all the livers of the rats with cirrhosis. We conclude that there is increased formation of S-nitrosothiols and nitrotyrosine in biliary cirrhosis, and this is markedly upregulated during endotoxemia. © 2001 Elsevier Science Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0891-5849(01)00647-5 | |
dc.source | Scopus | |
dc.subject | Bile duct ligation | |
dc.subject | Cirrhosis | |
dc.subject | Clearance | |
dc.subject | Endotoxemia | |
dc.subject | Experimental study | |
dc.subject | Free radicals | |
dc.subject | Half-life | |
dc.subject | LPS | |
dc.subject | Nitrotyrosine | |
dc.subject | S-nitrosoalbumin | |
dc.subject | S-nitrosothiols | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1016/S0891-5849(01)00647-5 | |
dc.description.sourcetitle | Free Radical Biology and Medicine | |
dc.description.volume | 31 | |
dc.description.issue | 6 | |
dc.description.page | 790-798 | |
dc.description.coden | FRBME | |
dc.identifier.isiut | 000171124100011 | |
Appears in Collections: | Staff Publications |
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