Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/38177
DC Field | Value | |
---|---|---|
dc.title | Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes | |
dc.contributor.author | Wang, W.J. | |
dc.contributor.author | Oates, C.G. | |
dc.contributor.author | Powell, A.D. | |
dc.date.accessioned | 2013-06-05T09:47:31Z | |
dc.date.available | 2013-06-05T09:47:31Z | |
dc.date.issued | 1996 | |
dc.identifier.citation | Wang, W.J., Oates, C.G., Powell, A.D. (1996). Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes. Bioresource Technology 55 (1) : 55-61. ScholarBank@NUS Repository. | |
dc.identifier.issn | 09608524 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/38177 | |
dc.description.abstract | Raw sage starch and sage starch pretreated by heating at 60°C for 2 hours in sodium acetate buffer (pH 3.5) were hydrolysed using commercial glucoamylase-AMG (EC 3.2.1.3), α-amylases-BAN, Fungamyl and Termamyl (EC 3.2.1.1), debranching amylase-Promozyme (EC 3.2.1.41), and their mixtures in sodium acetate buffer, pH 5.0 at 35°C. Raw sage starch was a poor substrate for enzyme action compared to corn and tapioca starches tested under the same conditions, although pretreating the starch increased the extent and rate of hydrolysis. A strong synergism between glucoamylase and α-amylase on the hydrolysis of both untreated and pretreated sage starch was observed. The hydrolysis products were characterized by high-performance size-exclusion chromatography (HPSEC). The total carbohydrate concentration of hydrolysed sage starch decreased but the amylose and amylopectin ratios in the residues remained the same. | |
dc.description.abstract | Raw sago starch and sago starch pretreated by heating at 60°C for 2 hours in sodium acetate buffer (pH 3.5) were hydrolysed using commercial glucoamylase-AMG (EC 3.2.1.3), α-amylases-BAN, Fungamyl and Termamyl (EC 3.2.1.1), debranching amylase-Promozyme (EC 3.2.1.41), and their mixtures in sodium acetate buffer, pH 5.0 at 35°C. Raw sago starch was a poor substrate for enzyme action compared to corn and tapioca starches tested under the same conditions, although pretreating the starch increased the extent and rate of hydrolysis. A strong synergism between glucoamylase and α-amylase on the hydrolysis of both untreated and pretreated sago starch was observed. The hydrolysis products were characterized by high-performance size-exclusion chromatography (HPSEC). The total carbohydrate concentration of hydrolysed sago starch decreased but the amylose and amylopectin ratios in the residues remained the same. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0960-8524(95)00132-8 | |
dc.publisher | Elsevier Science Ltd | |
dc.source | Scopus | |
dc.subject | Amylase | |
dc.subject | Glucoamylase | |
dc.subject | Raw starch hydrolysis | |
dc.subject | Sage starch | |
dc.subject | Starch oligosaccharide profiles | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.sourcetitle | Bioresource Technology | |
dc.description.volume | 55 | |
dc.description.issue | 1 | |
dc.description.page | 55-61 | |
dc.description.coden | BIRTE | |
dc.identifier.isiut | A1996UH83200004 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.