Please use this identifier to cite or link to this item: https://doi.org/10.1016/0005-2728(93)90212-X
Title: Effect of buffers and osmolality on anion uniport across the mitochondrial inner membrane
Authors: Ng, L.T. 
Selwyn, M.J. 
Choo, H.L.
Keywords: anion channel
matrix volume
mitochondrial inner membrane
mitochondrion
zwitterionic buffer
Issue Date: 1993
Source: Ng, L.T., Selwyn, M.J., Choo, H.L. (1993). Effect of buffers and osmolality on anion uniport across the mitochondrial inner membrane. Biochimica et Biophysica Acta - Bioenergetics 1143 (1) : 29-37. ScholarBank@NUS Repository. https://doi.org/10.1016/0005-2728(93)90212-X
Abstract: The effects of buffers and osmolality of the suspending medium on the pH-dependent anion uniport across the inner membrane of isolated rat liver mitochondria have been studied using the light scattering technique to measure passive osmotic swelling. In contrast to some other transport processes the rates of entry of chloride and other anions via the anion-conducting channel decreased steeply with increasing solute concentration. This effect appears to be a result of increased osmolality or decreased matrix volume rather than inhibition by the anion since it was also produced by increasing the osmolality by addition of non-penetrant solutes. The effects of some pH buffers on the mitochondrial anion-conducting channel were also investigated. Some zwitterionic buffers had little effect other than that produced by increasing osmolality but Tricine, Popso and Caps produced marked additional inhibition of anion uniport and several other zwitterionic buffers were also inhibitory. The correlation between increased anion conductivity and increased matrix volume supports the proposal that this channel functions in regulation of the volume of the mitochondrial matrix.
Source Title: Biochimica et Biophysica Acta - Bioenergetics
URI: http://scholarbank.nus.edu.sg/handle/10635/38104
ISSN: 00052728
DOI: 10.1016/0005-2728(93)90212-X
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

2
checked on Dec 5, 2017

WEB OF SCIENCETM
Citations

2
checked on Dec 5, 2017

Page view(s)

72
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.