Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.freeradbiomed.2004.03.018
Title: Mechanism of cell death induced by an antioxidant extract of Cratoxylum cochinchinense (YCT) in Jurkat T cells: The role of reactive oxygen species and calcium
Authors: Tang, S.Y.
Whiteman, M. 
Jenner, A. 
Peng, Z.F. 
Halliwell, B. 
Keywords: balcalein monohydrate
BHT
BL
BM
bromoenol lactone
butylated hydroxytoluene
Ca2+influx
DMEM
Dulbecco's modified Eagle's medium
Free radicals
Mitochondrial depolarization
Oxidative stress
Plasma membrane redox system
Potassium ferricyanide
Issue Date: 2004
Source: Tang, S.Y.,Whiteman, M.,Jenner, A.,Peng, Z.F.,Halliwell, B. (2004). Mechanism of cell death induced by an antioxidant extract of Cratoxylum cochinchinense (YCT) in Jurkat T cells: The role of reactive oxygen species and calcium. Free Radical Biology and Medicine 36 (12) : 1588-1611. ScholarBank@NUS Repository. https://doi.org/10.1016/j.freeradbiomed.2004.03.018
Abstract: YCT is a semipurified extract from Cratoxylum cochinchinense that has antioxidant properties and contains mostly mangiferin. We show here that YCT is selectively toxic to certain cell types and investigate the mechanisms of this toxicity in Jurkat T cells. By flow cytometric analyses, we show that YCT causes intense oxidative stress and a rise in cytosolic Ca2+. This is followed by a rise in mitochondrial Ca2+, release of cytochrome c, collapse of Δψm, a fall in ATP levels, and eventually cell death. The mechanism(s) of intense oxidative stress may involve a plasma membrane redox system, as cell death is inhibited by potassium ferricyanide. Cell death has some features of apoptosis (propidium iodide staining, externalization of phosphatidylserine, limited caspase-3 and -9 activities), but there was no internucleosomal DNA fragmentation. © 2004 Elsevier Inc. All rights reserved.
Source Title: Free Radical Biology and Medicine
URI: http://scholarbank.nus.edu.sg/handle/10635/38099
ISSN: 08915849
DOI: 10.1016/j.freeradbiomed.2004.03.018
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

17
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

17
checked on Nov 1, 2017

Page view(s)

72
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.