Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/37888
Title: Cell Cycle Lipidomics
Authors: LIM JING YAN
Keywords: cell cycle, G2/M arrest, acyl-coA cholesterol acyltransferase ACAT1, cholesterol ester, breast cancer, lipidomics
Issue Date: 11-Jan-2013
Source: LIM JING YAN (2013-01-11). Cell Cycle Lipidomics. ScholarBank@NUS Repository.
Abstract: Regulation of cell cycle is crucial for cell survival and function. Dysregulated cell division can cause many diseases, including cancer. How cellular lipid changes affect the cell cycle and vice versa have become an increasingly important question. Here, lipid composition of cells in different cell cycle stages was studied using mass spectrometry-based lipidomics approaches. Two complementing synchronization methods (aphidicolin and nocodazole) were applied on two cell lines (HeLa and MCF7). In terms of overall cellular lipid composition, all lipid classes, except cholesterol esters, did not show significant changes in the cell cycle. Levels of cholesterol esters measured were generally higher in G2/M cells. Prolonged G2/M phase in ACAT1 knocked-down cells which had lower amount of cholesterol esters supported the observation. Cholesterol esters levels were also found to be higher in breast tumour tissues compared to control. Overall, our findings indicate that cholesterol esters are crucial in G2/M progression and possibly, tumorigenesis.
URI: http://scholarbank.nus.edu.sg/handle/10635/37888
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Lim Jing Yan_Cell cycle lipidomics_thesis_2013.pdf2.28 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

182
checked on Dec 11, 2017

Download(s)

282
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.