Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/37887
Title: A study on premature segregation of unreplicated chromosomes
Authors: KHONG JENN HUI
Keywords: CDC6, G1-M checkpoint, premature chromosome segregation, CDC34, premature onset of mitosis
Issue Date: 3-May-2011
Source: KHONG JENN HUI (2011-05-03). A study on premature segregation of unreplicated chromosomes. ScholarBank@NUS Repository.
Abstract: Equal partitioning of the duplicated chromosomes to two daughter cells during cell division is a highly coordinated process and is initiated only after the completion of DNA synthesis. However, this strict order of execution breaks down in CDC6 deficient cells. Cdc6, an evolutionarily conserved protein, is required for the assembly of pre-replicative complexes (pre-RCs) and is essential for the initiation of DNA replication. Yeast cells lacking Cdc6 function, though unable to enter S phase, proceed to undergo `reductional anaphase? during which they segregate the unreplicated chromosomes and lose viability rapidly. It is thought that this extreme form of genomic instability in cdc6 cells is due to inactivation of a pre-RC based, Cdc6-dependent checkpoint mechanism that, during normal cell cycle, inhibits premature onset of mitosis until pre-RC is assembled. Here we show that the segregation of unreplicated chromosomes in Cdc6 deficient cells is caused not by precocious initiation of mitosis but by deregulation of the spindle requiring the activity of ubiquitin conjugating enzyme Cdc34.
URI: http://scholarbank.nus.edu.sg/handle/10635/37887
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