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Title: Co-culture-based Osteochondral Tissue Engineering
Keywords: BMSCs, Scaffold, Interface
Issue Date: 17-Aug-2012
Citation: CHEN KELEI (2012-08-17). Co-culture-based Osteochondral Tissue Engineering. ScholarBank@NUS Repository.
Abstract: The regeneration of whole osteochondral constructs with a physiological structure has been a significant issue, both clinically and academically. An optimal method that can regenerate multilayered tissue structure is needed. In this study, several co-culture methods were designed and investigated their effectiveness for generating the osteochondral interface and multilayered structure in vitro. Rabbit bone marrow stromal cells (BMSCs) and silk/RADA peptide scaffold were used in this study. The study was grouped into four stages: (i) design and development of the scaffold and osteochondral interface formation by 2D-3D co-culture system, (ii) development 3D-3D co-culture system, (iii) the development of static co-culture wells, and (iv) three-chamber bioreactor. Result revealed that osteogenic BMSCs/osteoblasts and chondrogenic BMSCs affected each other in this co-culture system and induced the formation of the osteochondral interface. Moreover, this system provided a possible approach for generating multilayered osteochondral constructs.
Appears in Collections:Ph.D Theses (Open)

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