Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/37743
Title: DENGUE VIRUS INFECTION UPREGULATES EXPRESSION OF CRACC, A RECEPTOR HAVING INNATE IMMUNO-MODULATORY FUNCTIONS
Authors: LIM SAY LIANG, DANIEL
Keywords: Dengue, Natural Killer Cells, Dendritic Cells, CRACC
Issue Date: 21-Jan-2013
Source: LIM SAY LIANG, DANIEL (2013-01-21). DENGUE VIRUS INFECTION UPREGULATES EXPRESSION OF CRACC, A RECEPTOR HAVING INNATE IMMUNO-MODULATORY FUNCTIONS. ScholarBank@NUS Repository.
Abstract: The innate immune response may influence dengue severity. NK cells are innate lymphocytes involved in antiviral defence. In this study, naive human NK cells co-cultured with dengue virus (DenV)-infected monocyte-derived dendritic cells (DCs) produced IFN-g and lysed infected cells. Type I IFNs and TNF-a produced by DenV-infected DCs were crucial, since their combined neutralization completely abolished the IFN-g response in these co-cultures. Furthermore, both cytokines acted in an autocrine manner to upregulate CRACC, a homotypic activating ligand/receptor for NK cells, on infected and bystander DCs. NK cells also recognized infected DCs through CRACC, as masking of the molecule during co-culture partially reduced the NK cell IFN-g response. Additionally, CRACC ligation suppressed production of IL-6, IL-8 and IL-12p70 in DCs challenged with LPS and IFN-g. Together, our study demonstrates synergy between type I IFNs and TNF-a in triggering NK cell anti-DenV responses and defines a role for CRACC in innate immunity.
URI: http://scholarbank.nus.edu.sg/handle/10635/37743
Appears in Collections:Ph.D Theses (Open)

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