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Title: Therapeutics and drug-designing against infectious diseases using synthetic enzymology
Keywords: Infectious diseases, Synthetic enzymology, Suprafamily of enzymes, Orotidine Monophosphate Decarboxylase , Keto gulonate phosphate decarboxylase
Issue Date: 2-Feb-2012
Source: AISHWARYA.S (2012-02-02). Therapeutics and drug-designing against infectious diseases using synthetic enzymology. ScholarBank@NUS Repository.
Abstract: Understanding the emergence of disease and the pathogenesis of multi-drug resistant microorganisms is significant to develop anti-infectives. In this project we endeavored to develop an antimicrobial drug against a Shikimate pathway enzyme, EfaroB and the Plasmodium?s OMPDC enzyme. Initially, EFaroB was expressed, purified and tested for its activity. Nearly 10 enzymes were subsequently chosen to clone, express and purify, to be employed as modifying enzymes for combinatorial biosynthesis. We attempted to decipher the OMPDC-catalyzed mechanism on KGPDC?s scaffold, to understand its mechanism. Mutations were performed on KGPDC orthologues based on the differing key residues between KGPDC and OMPDC enzymes followed by complementation growth studies on the KGPDC mutants. Finally, we tried to chance upon the disparity between the human and Plasmodium?s OMPDCs, for the rational design of an inhibitor against Plasmodium OMPDC, which might have minimal side-effects on the human host, and we noted few distinct key residues as targets.
Appears in Collections:Master's Theses (Open)

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