Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/37523
Title: DEVELOPMENT OF LIVING COLOR TRANSGENIC MEDAKA LINES FOR BIOMONITORING AQUATIC CONTAMINATION
Authors: NG HWEE BOON GRACE
Keywords: Ac/Ds, heat shock 70, cytochrome P450 1a, transgenic medaka, heavy metal, polycyclic aromatic hydrocarbon
Issue Date: 1-Oct-2012
Source: NG HWEE BOON GRACE (2012-10-01). DEVELOPMENT OF LIVING COLOR TRANSGENIC MEDAKA LINES FOR BIOMONITORING AQUATIC CONTAMINATION. ScholarBank@NUS Repository.
Abstract: Here we report the generation of two biomonitoring transgenic medaka lines using a stress-inducible promoter, hsp70, and xenobiotic inducible promoter, cyp1a, to express the GFP reporter gene and these two transgenic lines are Tg(hsp70:gfp) and Tg(cyp1a:gfp) respectively. A fairly novel transposon tool in fish transgenesis, maize Ac/Ds, was used to aid the generation of these transgenic medaka. High germline transmission rates and classic features of Ac/Ds transposon system were observed, hence demonstrating the high efficiency of Ac/Ds to aid transgene integration in medaka. Both Tg(hsp70:gfp) and Tg(cyp1a:gfp) medaka were exposed to various chemicals to determine their inducibility and sensitivity. Tg(hsp70:gfp) responds mainly to heavy metals such as mercury, arsenic and cadmium while Tg(cyp1a:gfp) responds mainly to polycyclic aromatic hydrocarbon (PAH) and dioxin groups such as 3-methylcholanthrene, benzo[a]pyrene and 2,3,7,8-tetrachlorodibenzodioxin. These observations indicate the feasibility of using Tg(hsp70:gfp) as indicator for heavy metal contamination and Tg(cyp1a:gfp) as indicator for PAH contamination.
URI: http://scholarbank.nus.edu.sg/handle/10635/37523
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
NgHBG.pdf3.76 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

125
checked on Dec 18, 2017

Download(s)

15
checked on Dec 18, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.