Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0090-8258(03)00396-2
Title: Resistance to Fas-mediated cell death in BeWo and NJG choriocarcinoma cell lines: Implications in immune privilege
Authors: Rajashekhar, G. 
Loganath, A. 
Roy, A.C. 
Mongelli, J.M. 
Keywords: Apoptosis
cFLIP
Cycloheximide
FasL
Immune privilege
Issue Date: 2003
Source: Rajashekhar, G., Loganath, A., Roy, A.C., Mongelli, J.M. (2003). Resistance to Fas-mediated cell death in BeWo and NJG choriocarcinoma cell lines: Implications in immune privilege. Gynecologic Oncology 91 (1) : 89-100. ScholarBank@NUS Repository. https://doi.org/10.1016/S0090-8258(03)00396-2
Abstract: Objective. An immune privileged site occurs when the allogenic tissue grafts have the propensity for prolonged survival in the host tissue. In this context, the survival and proliferation of malignant trophoblasts in the gravid uterus are currently unclear. In a previous study, we documented that Fas and FasL are coexpressed in choriocarcinoma [Gynecol. Oncol. (2003)]. This study was conducted to examine the role of the Fas/FasL pathway in immune privilege of BeWo and NJG choriocarcinoma cells in culture. Methods. The ability of anti-Fas mAb (CH-11) to sensitize choriocarcinoma cell lines to Fas-mediated cytotoxicity was assessed by MTT assays. Coculture experiments with Fas-sensitive Jurkat cells were used to demonstrate functional FasL from choriocarcinoma. RT-PCR was used to assess the expression of cFLIP. Results. The mean cell viability of BeWo and NJG cells declined to about 58 and 63% compared to controls after 72 h of culture in the presence of anti-Fas mAb (CH-11) while the Fas-sensitive Jurkat cells showed viability of only 10%. This resistance to Fas-mediated apoptosis in choriocarcinoma cells is reversed in the presence of cycloheximide (0.5 μg/ml) which further decreased the viability to 36 and 32%, respectively, at a dose of 300 ng/ml (P < 0.05). The observed resistance to Fas-mediated apoptosis therefore could be attributed to the short-lived endogenous inhibitor, cFLIP as demonstrated by the RT-PCR technique. In coculture experiments, FasL from choriocarcinoma cells induced apoptosis in the Fas-sensitive Jurkat cells, thereby indicating the capacity to evade immune attack. Conclusion. Decreased sensitivity to Fas-mediated apoptosis and counterattacking the lymphocytes may impart immune privilege in these malignant trophoblasts for prolonged survival in the host. © 2003 Elsevier Inc. All rights reserved.
Source Title: Gynecologic Oncology
URI: http://scholarbank.nus.edu.sg/handle/10635/36942
ISSN: 00908258
DOI: 10.1016/S0090-8258(03)00396-2
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

6
checked on Dec 11, 2017

WEB OF SCIENCETM
Citations

6
checked on Dec 11, 2017

Page view(s)

96
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.