Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/36543
Title: Establishment of transposon mutagenesis for Mycobacterium smegmatis
Authors: NGUYEN THUY KHANH
Keywords: tuberculosis, drug targets, transposon mutagenesis, Mycobacterium smegmatis, EZ-Tn5 transposon, rescue cloning
Issue Date: 27-Dec-2012
Source: NGUYEN THUY KHANH (2012-12-27). Establishment of transposon mutagenesis for Mycobacterium smegmatis. ScholarBank@NUS Repository.
Abstract: Tuberculosis (TB) remains a major global health problem despite the availability of effective chemotherapy. This is largely a result of the emergence of drug-resistant strains of M. tuberculosis and the poor compliance of the long treatment of TB that requires therapy of multiple drugs. Thus, it is urgently needed to discover new targets for more effective anti-mycobacterial drugs. In order to find new target, it is needed to understand the biology of the pathogen and function of its genes. Transposon mutagenesis is an excellent tool to dissect the genome of the organism for uncovering gene function. The non-pathogenic and fast growing M. smegmatis is a commonly used model for surrogate-host genetic analysis of mycobacterial pathogens. The main aim of the project is to establish a transposon mutagenesis method for M. smegmatis mc2 155 using the EZ-Tn5 transposome system. The ?rescue cloning? method was applied to identify the transposon-disrupted gene.
URI: http://scholarbank.nus.edu.sg/handle/10635/36543
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