Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/36464
Title: FUNCTIONAL ANALYSIS OF ANAPLASTIC LYMPHOMA KINASE DURING EMBRYONIC NEUROGENESIS IN ZEBRAFISH
Authors: YAO SHENG
Keywords: anaplastic lymphoma kinase, midkine, neurogenesis, proliferation, apoptosis, zebrafish
Issue Date: 17-Aug-2012
Source: YAO SHENG (2012-08-17). FUNCTIONAL ANALYSIS OF ANAPLASTIC LYMPHOMA KINASE DURING EMBRYONIC NEUROGENESIS IN ZEBRAFISH. ScholarBank@NUS Repository.
Abstract: The orphan receptor tyrosine kinase gene Anaplastic Lymphoma Kinase (ALK) was initially discovered as an oncogene. ALK kinase over-activation has been associated with human cancers, most importantly neuroblastoma. However, little is known about ALK¿s endogenous function in development. I identified an alk ortholog in zebrafish and used this model species to analyze the embryonic function of alk. Zebrafish alk is highly expressed in the central nervous system (CNS) during embryonic development. Constitutive overexpression of non-mutated alk in a heat shock inducible transgenic zebrafish line resulted in ectopic MAPK activation, elevated cell proliferation, disruption of neurogenesis patterns, as well as mis-positioning of differentiated neurons. Loss-of-function in alk by, Morpholino knock-down and a small molecule inhibitor compromised neuronal differentiation and neuron survival in the CNS, but left progenitor formation intact. Also Midkine, a putative ligand for Alk and receptor protein tyrosine phosphatase zeta (RPTPz), a known Alk interactor, are briefly discussed.
URI: http://scholarbank.nus.edu.sg/handle/10635/36464
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
YaoS.pdf8.54 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

153
checked on Dec 11, 2017

Download(s)

23
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.