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Title: Microparticle Array on Gel Microstructure Chip for Multiplexed Biochemical Assays
Keywords: microparticle array, gel microstructure, multiplexed biochemical assay, microfluidics, capillary system
Issue Date: 16-Aug-2012
Citation: ZHU QINGDI (2012-08-16). Microparticle Array on Gel Microstructure Chip for Multiplexed Biochemical Assays. ScholarBank@NUS Repository.
Abstract: In this PhD work, novel polyacrylamide gel microstructures have been developed for the assembly of microparticle arrays for multiplexed biochemical assays. The fabrication of these gel microstructures requires short process time, low material cost and minimal equipment. Based on these gel microstructures, three types of chips are fabricated for various multiplexed biochemical assays. The first type of chip consists of 40 gel pad arrays with PEG micropillar rings around them. This gel pad array chip is used for multiplexed immunoassay for proteins in serum with 1 ?l sample volume. The second type of chip is to be integrated with microfluidics. With this chip, the first integration of immunoassay and enzymatic assay into one microfluidic assay is demonstrated. On the third type of chip, the first integration of a microparticle array into a capillary system for biochemical assays has been demonstrated. The chip enables the immunoassay to be done in 10 min with no repeated manual washing steps involved. This novel microparticle array platform could be a useful and more accessible research tool for common biolabs to customize their own microparticle arrays for multiplexed and quantitative bioanalysis.
Appears in Collections:Ph.D Theses (Open)

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