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Title: Engineered Poly(L-Lactic acid) - Based Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells
Keywords: Biomimetic, Bone, Electrospinning, Nanofibers, MSCs, PLLA
Issue Date: 21-Aug-2012
Citation: NGUYEN THI HIEN LUONG (2012-08-21). Engineered Poly(L-Lactic acid) - Based Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells. ScholarBank@NUS Repository.
Abstract: The main objective of this thesis is to develop an ideal bone graft material which should possess osteoconductive, osteogenic and osteoinductive properties without using expensive growth factors. Poly(L-lactic acid) (PLLA), type I collagen (Col) and hydroxyapatite (HA) ? biocompatible and biodegradable materials ? were employed. These materials were fabricated into nanocomposites consisted of PLLA/Col nanofibers and nano-HA crystals to mimic native bone extracellular matrix (ECM). We hypothesized that the biomimetic scaffolds with nanotopography and a sustainable local supply of calcium/phosphate (Ca/P) ions would effectively induce osteogenic differentiation of stem cells without the need for osteogenic solutes. The scaffolds were enriched with mesenchymal stem cells (MSCs) to provide the osteogenic property. Our in vitro and in vivo studies showed that PLLA/Col/HA nanocomposite enriched with MSCs was an ideal scaffold for bone regeneration which had osteoconductive, osteoinductive and osteogenic properties. Three-dimensional (3D) nanofibrous scaffolds with macroporous structure were then developed to further improve the migration and osteogenic differentiation ability of MSCs.
Appears in Collections:Ph.D Theses (Open)

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