Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/36141
Title: Vascularised Bone Tissue Engineering: Endothelial progenitor cells and human mesenchymal stem cells coculture in 3D honeycomb scaffolds and the effect of bi-rotational bioreactor and hypoxic microenvironment
Authors: LIU YUCHUN
Keywords: bone tissue engineering, bioreactor, coculture, hypoxia, stem cells, vascularisation
Issue Date: 17-Aug-2012
Source: LIU YUCHUN (2012-08-17). Vascularised Bone Tissue Engineering: Endothelial progenitor cells and human mesenchymal stem cells coculture in 3D honeycomb scaffolds and the effect of bi-rotational bioreactor and hypoxic microenvironment. ScholarBank@NUS Repository.
Abstract: Poor angiogenesis impairs bone regeneration, limiting the clinical translation of bone tissue engineered (BTE) grafts for the repair of large defects. In this project, vasculogenic endothelial progenitor cells (EPC) cocultured with osteogenic human fetal mesenchymal stem cells (hfMSC) potentiated its osteogenic differentiation in vitro through paracrine signalling and formed an in vitro vascular network in the three dimensional honeycomb polycaprolactone/tri-calcium phosphate composite scaffolds. Upon subcutaneous implantation, EPC/hfMSC showed enhanced vascularity and consequentially, improved osteogenicity in vivo. Biomechanical stimulation of EPC/hfMSC-grafts in a biaxial bioreactor resulted in increased cell viability, more robust bone formation and increased vasculogenesis compared to its implanted static-cultured coculture. In addition, the maintenance of hfMSC under continuous hypoxic microenvironment upon cell isolation demonstrated enhanced colony-forming ability and osteogenic potential. This thesis explores the use of a coculture of stem cells, in conjunction with bioresorbable scaffolds, bioreactor technologies and a low oxygen microenvironment for the generation of voluminous bone grafts that are capable of rapid vascularisation for facilitating bone repair.
URI: http://scholarbank.nus.edu.sg/handle/10635/36141
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
05-07 Chap. Liu Yuchun PhD Thesis 2012.pdf3.96 MBAdobe PDF

OPEN

NoneView/Download
01-04 Chap. Liu Yuchun PhD Thesis 2012.pdf10.36 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

355
checked on Jan 19, 2018

Download(s)

733
checked on Jan 19, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.