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Title: | Vascularised Bone Tissue Engineering: Endothelial progenitor cells and human mesenchymal stem cells coculture in 3D honeycomb scaffolds and the effect of bi-rotational bioreactor and hypoxic microenvironment | Authors: | LIU YUCHUN | Keywords: | bone tissue engineering, bioreactor, coculture, hypoxia, stem cells, vascularisation | Issue Date: | 17-Aug-2012 | Citation: | LIU YUCHUN (2012-08-17). Vascularised Bone Tissue Engineering: Endothelial progenitor cells and human mesenchymal stem cells coculture in 3D honeycomb scaffolds and the effect of bi-rotational bioreactor and hypoxic microenvironment. ScholarBank@NUS Repository. | Abstract: | Poor angiogenesis impairs bone regeneration, limiting the clinical translation of bone tissue engineered (BTE) grafts for the repair of large defects. In this project, vasculogenic endothelial progenitor cells (EPC) cocultured with osteogenic human fetal mesenchymal stem cells (hfMSC) potentiated its osteogenic differentiation in vitro through paracrine signalling and formed an in vitro vascular network in the three dimensional honeycomb polycaprolactone/tri-calcium phosphate composite scaffolds. Upon subcutaneous implantation, EPC/hfMSC showed enhanced vascularity and consequentially, improved osteogenicity in vivo. Biomechanical stimulation of EPC/hfMSC-grafts in a biaxial bioreactor resulted in increased cell viability, more robust bone formation and increased vasculogenesis compared to its implanted static-cultured coculture. In addition, the maintenance of hfMSC under continuous hypoxic microenvironment upon cell isolation demonstrated enhanced colony-forming ability and osteogenic potential. This thesis explores the use of a coculture of stem cells, in conjunction with bioresorbable scaffolds, bioreactor technologies and a low oxygen microenvironment for the generation of voluminous bone grafts that are capable of rapid vascularisation for facilitating bone repair. | URI: | http://scholarbank.nus.edu.sg/handle/10635/36141 |
Appears in Collections: | Ph.D Theses (Open) |
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05-07 Chap. Liu Yuchun PhD Thesis 2012.pdf | 3.96 MB | Adobe PDF | OPEN | None | View/Download | |
01-04 Chap. Liu Yuchun PhD Thesis 2012.pdf | 10.36 MB | Adobe PDF | OPEN | None | View/Download |
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