Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/36024
Title: PROTEOMICS BASED ANALYSIS IDENTIFIED CACYBP AS A CANDIDATE BIOMARKER FOR COLORECTAL CANCER METASTASIS
Authors: DIPANJANA GHOSH
Keywords: Colorectal Cancer, Metastasis, iTRAQ, CacyBP, Proteomics, Biomarker
Issue Date: 15-Aug-2012
Source: DIPANJANA GHOSH (2012-08-15). PROTEOMICS BASED ANALYSIS IDENTIFIED CACYBP AS A CANDIDATE BIOMARKER FOR COLORECTAL CANCER METASTASIS. ScholarBank@NUS Repository.
Abstract: This research aims to identify potential biomarker candidate(s) for CRC metastasis while performing a detailed investigation of its underlying mechanisms. Preliminary proteomics based experiments on CRC cell lines of different metastatic potentials provided an overall proteome signature of CRC metastasis as well as showed good correlation of intra-cellular CacyBP (Calcyclin Binding Protein, causes degradation of ß-catenin) level with metastasis. Preliminary functional studies suggested decreased cellular adhesion due to enhanced degradation of ß-catenin as one of the causes behind this fact. Enhanced level of CacyBP and its direct association with CRC metastasis was further reinforced by the metastatic signature presented by CacyBP-overexpressed primary and CacyBP-knockdown metastatic CRC cells. Subsequent iTRAQ based experiments on the whole cell lysate from these cells (CacyBP-overexpressed primary and CacyBP-knockdown metastatic CRC cells) confirmed that CacyBP enhances metastatic nature of CRC by various mechanisms such as increased endocytosis of integrins and its recycling to the leading edge of the cell, enhancing actin nucleation etc. In short this study proposed CacyBP as one of the potential candidate biomarker protein for CRC metastasis and also shed light on the possible mechanism by which this protein promotes CRC metastasis which contributes fruitfully in CRC research.
URI: http://scholarbank.nus.edu.sg/handle/10635/36024
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