Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/36004
Title: IDENTIFICATION OF MOUSE EMBRYONIC NEURAL STEM CELL SURFACE MARKERS
Authors: YU YUAN HONG
Keywords: mouse, neural stem cells, neurospheres, surface marker, C1qR1, LeX
Issue Date: 26-Jun-2012
Source: YU YUAN HONG (2012-06-26). IDENTIFICATION OF MOUSE EMBRYONIC NEURAL STEM CELL SURFACE MARKERS. ScholarBank@NUS Repository.
Abstract: Neural stem cells (NSCs) have yet to be definitively identified and isolated in vitro. To facilitate the isolation of NSCs it is essential that we identify a unique set of cell surface markers. Here, I performed multiplex single cell RT-PCR on mRNA from neurosphere derived cells with the aim of identifying novel cell surface markers for NSCs. Using this methodology we identified C1qR1 (CD93) as a potential cell surface marker for NSCs. C1qR1+ single cells propagated to form clonal neurospheres gave a 5.8% NSC frequency, 6-fold higher than unsorted cells, and higher secondary neurosphere formation. C1qR1 was expressed in vivo in the ventricular and subventricular zones of the brain, areas known to be enriched for NSCs. C1qR1+ selected cells from brain had greater neurosphere formation, multipotency and self-renewal capacity in primary cultures. Further, C1qR1+/LeX+ cells were enriched for NSCs by approximately 35-fold giving a 46% NSC frequency. C1qR1+/LeX+ cells expressed significantly higher levels of Hes1, Hes5, Musahsi1, nestin, Pax6, and Sox2 than unsorted cells. I conclude that C1qR1 is a novel marker for NSCs, and that along with LeX, can be used to select for a highly enriched NSC population.
URI: http://scholarbank.nus.edu.sg/handle/10635/36004
Appears in Collections:Ph.D Theses (Open)

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