Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35890
Title: Molecular analysis of the roles of yeast microtubule-associated genes in Agrobacterium-mediated transformation
Authors: CHEN ZIKAI
Keywords: microtubule, yeast, AMT, T-DNA, ARP6, FISH
Issue Date: 27-Jul-2012
Source: CHEN ZIKAI (2012-07-27). Molecular analysis of the roles of yeast microtubule-associated genes in Agrobacterium-mediated transformation. ScholarBank@NUS Repository.
Abstract: A. tumefaciens virulence machinery facilitating the transfer of T-DNA has been intensively investigated; however, the trafficking pathway of T-DNA inside eukaryotic cells is not well established.In this project, Saccharomyces cerevisiae is used as a research model to investigate the roles of yeast microtubule-associated genes in T-DNA trafficking inside cells, since previous studies have shown that T-complex can utilize a microtubule-based transport pathway to deliver T-DNA in vitro. To identify the host components involved in T-DNA transfer, 186 yeast knockout mutants of genes associated with microtubules were screened by Agrobacterium-mediated transformation (AMT). The results demonstrate that 16 genes associated with microtubules were important for the T-DNA transfer. In order to further understand the roles of microtubules in AMT process, the gene functions of ARP6 which significantly affects transformation efficiency were extensively investigated. Moreover, T-DNA trafficking was studied with a combination of genetic, biochemical, and bio-imaging approaches in this project.
URI: http://scholarbank.nus.edu.sg/handle/10635/35890
Appears in Collections:Ph.D Theses (Open)

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