Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35822
Title: Molecular Biology of the lung and kidney of the African lungfish, Protopterus annectens, during three phases of aestivation: cystic fibrosis transmembrane conductance regulator, gulonolactone oxidase and p53
Authors: CHING BIYUN
Keywords: cftr, gulo, antioxidant, ascorbic acid
Issue Date: 18-Jun-2012
Source: CHING BIYUN (2012-06-18). Molecular Biology of the lung and kidney of the African lungfish, Protopterus annectens, during three phases of aestivation: cystic fibrosis transmembrane conductance regulator, gulonolactone oxidase and p53. ScholarBank@NUS Repository.
Abstract: The African lungfish, Protopterus annectens, possesses lungs in addition to gills and is able to aestivate on land for extended periods. Cystic fibrosis transmembrane conductance regulator (cftr), gulonolactone oxidase (gulo) and p53 were cloned and sequenced from P. annectens. Results reveal novel findings of multiple cftr/Cftr forms in the lungs and gills of P. annectens, suggesting that non-coding DNA may not be the only reason to explain the huge genome of P. annectens. In addition, gulo/Gulo was expressed in multiple tissues and not only in the kidney. It is probable that gulo expression in multiple tissues was essential to produce ascorbate in situ for antioxidative defence during aestivation. The cDNA sequence of p53 from the lung of P. annectens was uniquely longer than those of other species. In the kidney, changes in p53 mRNA levels indicated the occurrence of apoptotic events to facilitate cell and tissue reconstruction during the induction and arousal phases of aestivation.
URI: http://scholarbank.nus.edu.sg/handle/10635/35822
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
ChingBY.pdf10.46 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

262
checked on Dec 11, 2017

Download(s)

582
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.