Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35644
Title: FUNCTIONAL CHARACTERIZATION OF AUGMENTER OF LIVER REGENERATION (ALR) IN ZEBRAFISH LIVER DEVELOPMENT
Authors: LI YAN
Keywords: augmenter of liver regeneration (alr), liver development, zebrafish, sulfhydryl oxidase, artificial microRNA, knockdown
Issue Date: 2-Jul-2012
Source: LI YAN (2012-07-02). FUNCTIONAL CHARACTERIZATION OF AUGMENTER OF LIVER REGENERATION (ALR) IN ZEBRAFISH LIVER DEVELOPMENT. ScholarBank@NUS Repository.
Abstract: Augmenter of Liver Regeneration (ALR) is a hepatotrophic growth factor that specifically stimulates hepatocyte proliferation and promotes liver regeneration after liver damage or partial hepatectomy. It is also a sulfhydryl oxidase carrying out fundamental functions by facilitating protein disulfide bond formation. However, whether ALR also plays a role during vertebrate hepatogenesis is unknown. In this work, the function of alr in liver organogenesis was investigated in the zebrafish model. In part I, I demonstrated that zebrafish Alr is a FAD-linked sulfhydryl oxidase localized in the mitochondria and cytosol. The alr is required for embryonic liver growth by promoting hepatocyte proliferation, using mechanisms that are dependent as well as independent of its sulfhydryl oxidase activity. In part II, temporally- and spatially-controllable knockdown of alr was achieved in stable transgenic zebrafish, by combining the liver-specific promoter controlled LexPR-inducible gene expression system with the artificial miRNA-mediated knockdown system.
URI: http://scholarbank.nus.edu.sg/handle/10635/35644
Appears in Collections:Ph.D Theses (Open)

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