Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35584
Title: SORTING NEXIN 3 REGULATES PHAGOCYTOSIS AND CROSS-PRESENTATION IN DENDRITIC CELLS
Authors: CHUA RONG YUAN, RAY
Keywords: SNX-3, Phagocytosis, Cross-presentation, DC,
Issue Date: 14-May-2012
Source: CHUA RONG YUAN, RAY (2012-05-14). SORTING NEXIN 3 REGULATES PHAGOCYTOSIS AND CROSS-PRESENTATION IN DENDRITIC CELLS. ScholarBank@NUS Repository.
Abstract: Dendritic cells (DC) are the key cell type that carries out phagocytosis and cross-presentation. A well-regulated membrane/protein trafficking network is integral to phagocytosis and cross-presentation. Sorting nexin 3 (SNX-3) is an established regulator in membrane/protein trafficking. Intriguingly, the roles of SNX-3 along the phagocytic pathway and cross-presentation have not been investigated. Our results show that silencing of SNX-3 enhanced phagocytosis in DC. Furthermore, SNX-3 competes with a larger PI(3)P lipid-binding protein, EEA-1, in the recruitment to membranes. Hence, SNX-3 may negatively regulates phagocytosis in DC by modulating the recruitment of larger PI(3)P lipid-binding proteins such as EEA-1 to phagosomal membranes. In addition, SNX-3 knockdown DC showed an increase in surface expression of H-2K/D and H-2Kb/SIINFEKL that explained the increased cross-presentation in DC. Such modulation of MHC I and peptide-MHC I complexes (pMHC I) surface expression is likely linked to SNX-3¿s role in regulating phagocytosis and early endosomal sorting and recycling.
URI: http://scholarbank.nus.edu.sg/handle/10635/35584
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01_Title Page_Declaration_Acknowledgements.pdf37.13 kBAdobe PDF

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02_Table of contents.pdf119.68 kBAdobe PDF

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03_List of tables and figures.pdf91.21 kBAdobe PDF

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04_ Abbreviations.pdf88.15 kBAdobe PDF

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05_Summary.pdf39.85 kBAdobe PDF

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06_Thesis Chapter 1.pdf1.47 MBAdobe PDF

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07_Thesis Chapter 2.pdf228.12 kBAdobe PDF

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08_Thesis Chapter 3.pdf4.41 MBAdobe PDF

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10_Thesis Chapter 5.pdf278.89 kBAdobe PDF

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11_References.pdf114.55 kBAdobe PDF

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12_Appendices.pdf329.47 kBAdobe PDF

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13_Publications and conferences.pdf29.96 kBAdobe PDF

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