Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35552
Title: Effects of Combined Mechanical and Pulsed Electromagnetic Field Stimulations on the Osteogenesis of Bone Marrow Stem Cells
Authors: NG KIAN SIANG
Keywords: Pulsed electromagnetic field, bone marrow stem cells, osteogenesis, perfusion bioreactor, bone tissue engineering, silk scaffold
Issue Date: 15-Jun-2012
Source: NG KIAN SIANG (2012-06-15). Effects of Combined Mechanical and Pulsed Electromagnetic Field Stimulations on the Osteogenesis of Bone Marrow Stem Cells. ScholarBank@NUS Repository.
Abstract: The aim of the project was to investigate the effects of combining mechanical and pulsed electromagnetic field (PEMF) stimulations on the osteogenesis of bone marrow stem cells (BMSCs). To achieve this, a perfusion bioreactor integrated with PEMF stimulation apparatus was built. BMSCs were cultured on different culture systems and exposed to PEMF stimulation, starting from a static 2D culture plate model, to a static 3D scaffold model and finally a 3D scaffold housed in a dynamic bioreactor. Findings from the study showed that PEMF stimulation on 2D and 3D static models was able to increase the formation of mineralized extracellular matrix (ECM) over the control. Combining both PEMF and perfusion bioreactors did not lead to a synergistic production in mineralized ECM as hypothesized. Instead, the combined stimulation led to an earlier formation of mineralized ECM. These highlighted the potential of using combined stimulations to form better bone grafts faster.
URI: http://scholarbank.nus.edu.sg/handle/10635/35552
Appears in Collections:Ph.D Theses (Open)

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