Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35531
Title: SYSTEM INVESTIGATION OF LY303511-INDUCED SENSITIZATION OF HELA CELLS TO TRAIL-INDUCED APOPTOSIS: ROLE OF HYDROGEN PEROXIDE, PEROXYNITRITE, NITRIC OXIDE AND CALCIUM
Authors: SHI YUAN
Keywords: ordinary differential equation, Bayesian network, TRAIL, LY30, synergistic cell death,reactive oxygen species
Issue Date: 23-Mar-2012
Source: SHI YUAN (2012-03-23). SYSTEM INVESTIGATION OF LY303511-INDUCED SENSITIZATION OF HELA CELLS TO TRAIL-INDUCED APOPTOSIS: ROLE OF HYDROGEN PEROXIDE, PEROXYNITRITE, NITRIC OXIDE AND CALCIUM. ScholarBank@NUS Repository.
Abstract: We applied a computational system method to study the synergistic killing mechanism of LY30 and TRAIL. The project involves modeling synergistic effect based on previous studies and investigating the involvement of ROS in the synergistic effect. We firstly used mathematical modeling and ordinary differential equations to represent how LY30 and TRAIL individually affects HeLa cells, and to predict how the combined treatment achieves synergy. Model-based predictions are compared with in vitro experiments. We then investigated the source and dynamics of intracellular Reactive Oxygen Species to confirm the cause of cFLIP fluctuation by LY30. Our work shows that LY30 has complex effect on ROS, RNS and calcium, which exerts complex downstream effect such as non-monotonic effects on cFLIP-s. Our work also demonstrates that computational modeling can exploit kinetic data to identify novel effects, and to suggest which time intervals may have transient effects that require further sampling.
URI: http://scholarbank.nus.edu.sg/handle/10635/35531
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