Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35530
Title: EXACTIN-A SPECIFIC INHIBTOR OF 'FACTOR X' ACTIVATION BY EXTRINSIC TENASE COMPLEX ISOLATED FROM HEMACHATUS HAEMACHATUS VENOM
Authors: VALLERINTEAVIDE MAVELLI GIRISH
Keywords: Anticoagulant, Snake venom, Three-finger toxins, Factor X, Extrinsic tenase complex, Exactin
Issue Date: 20-Jan-2012
Source: VALLERINTEAVIDE MAVELLI GIRISH (2012-01-20). EXACTIN-A SPECIFIC INHIBTOR OF 'FACTOR X' ACTIVATION BY EXTRINSIC TENASE COMPLEX ISOLATED FROM HEMACHATUS HAEMACHATUS VENOM. ScholarBank@NUS Repository.
Abstract: Anticoagulants prevent the formation of unwanted clots that lead to heart attack and stroke resulting in a large number of deaths in developed countries. Currently available drugs have some drawbacks including their non-specific actions. Therefore novel anticoagulants that target specific steps in the coagulation pathway are being sought. We have been searching for new anticoagulants from snake venoms. Through activity-based screening, many anticoagulants targeting the extrinsic activation complex were identified from the venom of H. haemachatus. In the current study, functional and structural characterization of one of this anticoagulant protein, exactin was carried out. The anticoagulant protein was purified from the crude venom using a combination of gel filtration and reverse-phase chromatography and characterized by ESI-MS, CD spectroscopy and Edman degradation sequencing. Enzyme kinetic assays have shown that exactin inhibited the activation of FX specifically by the reconstituted extrinsic tenase complex with a novel mechanism.
URI: http://scholarbank.nus.edu.sg/handle/10635/35530
Appears in Collections:Ph.D Theses (Open)

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