Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35267
Title: METHOD FOR EXPRESSING SIALYLATED GLYCOPROTEINS IN MAMMALIAN CELLS AND CELLS THEREOF
Authors: WONG SOO CHING NIKI CECILIA 
YAP GEK SIM MIRANDA 
WANG I-CHYAU DANIEL 
Issue Date: 3-Dec-2009
Source: WONG SOO CHING NIKI CECILIA,YAP GEK SIM MIRANDA,WANG I-CHYAU DANIEL (2009-12-03). METHOD FOR EXPRESSING SIALYLATED GLYCOPROTEINS IN MAMMALIAN CELLS AND CELLS THEREOF. ScholarBank@NUS Repository.
Abstract: Methods and systems for producing glycoproteins having sialylated oligosaccharides are provided. The invention comprises the genetic engineered cells with a CMP-sialic acid transporter (CMP-SAT) gene so that the cells express the CMP-SAT protein or fragment thereof at an above endogenous levels. The increase in CMP-SAT expression allows for the increased transport of the CMP-sialic acid into the Golgi apparatus so as to obtain sialylation of glycoproteins at above endogenous levels. In particular, the methods and systems of the invention are useful for producing complex sialylated glycoproteins in mammalian cells of interest, for example Chinese Hamster Ovary (CHO) cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/35267
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