Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/35218
DC Field | Value | |
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dc.title | SINGLE-MOLECULE STUDIES OF DNA ORGANIZATION BY BACTERIAL ARCHITECTURAL PROTEINS | |
dc.contributor.author | LIN JIE | |
dc.date.accessioned | 2012-10-31T18:00:58Z | |
dc.date.available | 2012-10-31T18:00:58Z | |
dc.date.issued | 2012-04-04 | |
dc.identifier.citation | LIN JIE (2012-04-04). SINGLE-MOLECULE STUDIES OF DNA ORGANIZATION BY BACTERIAL ARCHITECTURAL PROTEINS. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/35218 | |
dc.description.abstract | The integration host factor is an abundant nucleoid-associated protein and an essential co-factor for the site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting biological importance of non-specific binding of IHF. However, it is unclear how non-specific DNA association contributes to DNA organization. Using single DNA manipulation and AFM imaging methods, we show distinct modes of non-specific DNA binding of IHF. Changes in KCl and IHF concentrations, as well as force dramatically influence the degree of DNA-bending. Moreover, IHF crosslinks DNA into highly compact DNA meshworks in the presence of magnesium at low concentration of monovalent-ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation. | |
dc.language.iso | en | |
dc.subject | Single DNA manipulation, Atomic Force Microscopy, Integration Host Factor, Nucleoid associated protein, magnetic tweezers, non-specific binding | |
dc.type | Thesis | |
dc.contributor.department | PHYSICS | |
dc.contributor.supervisor | YAN JIE | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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LinJ.pdf | 3.66 MB | Adobe PDF | OPEN | None | View/Download |
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