Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35218
Title: SINGLE-MOLECULE STUDIES OF DNA ORGANIZATION BY BACTERIAL ARCHITECTURAL PROTEINS
Authors: LIN JIE
Keywords: Single DNA manipulation, Atomic Force Microscopy, Integration Host Factor, Nucleoid associated protein, magnetic tweezers, non-specific binding
Issue Date: 4-Apr-2012
Source: LIN JIE (2012-04-04). SINGLE-MOLECULE STUDIES OF DNA ORGANIZATION BY BACTERIAL ARCHITECTURAL PROTEINS. ScholarBank@NUS Repository.
Abstract: The integration host factor is an abundant nucleoid-associated protein and an essential co-factor for the site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting biological importance of non-specific binding of IHF. However, it is unclear how non-specific DNA association contributes to DNA organization. Using single DNA manipulation and AFM imaging methods, we show distinct modes of non-specific DNA binding of IHF. Changes in KCl and IHF concentrations, as well as force dramatically influence the degree of DNA-bending. Moreover, IHF crosslinks DNA into highly compact DNA meshworks in the presence of magnesium at low concentration of monovalent-ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation.
URI: http://scholarbank.nus.edu.sg/handle/10635/35218
Appears in Collections:Ph.D Theses (Open)

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