Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/35204
Title: INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY
Authors: TEO LIN SHIN
Keywords: Fluorescence correlation spectroscopy, Drosophila melanogaster, Central nervous system, Alzheimer's disease, membrane fluidity, Ceramide
Issue Date: 16-May-2012
Source: TEO LIN SHIN (2012-05-16). INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY. ScholarBank@NUS Repository.
Abstract: The objective of our study is to apply biophysical fluorescence technique, i.e. fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) embryo to study plasma membrane dynamics. We showed that fluorescent proteins exhibited distinct diffusion properties depending on different subcellular locations. Then we altered the membrane lipid composition by genetic and pharmacological manipulations that should change membrane fluidity. The changes in membrane sphingolipid composition or microenvironment were reflected in the diffusion behavior of the membrane probes employed. To our knowledge, this is the first time that neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS. Our approach promises to shed light on the biophysical features of cellular membranes in fly mutants or disease models in which membrane dynamics or regulation of lipid composition may play a part in the development and pathogenesis of diseases.
URI: http://scholarbank.nus.edu.sg/handle/10635/35204
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
TeoLS.pdf2.09 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

202
checked on Dec 11, 2017

Download(s)

35
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.