Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34928
Title: Chimeric gene constructs for generation of fluorescent transgenic ornamental fish
Authors: GONG, ZHIYUAN 
HE, JIANGYAN 
JU, BENSHENG 
LAM, TOONG JIN 
XU, YANFEI 
YAN, TIE 
Issue Date: 22-Jul-2004
Source: GONG, ZHIYUAN,HE, JIANGYAN,JU, BENSHENG,LAM, TOONG JIN,XU, YANFEI,YAN, TIE (2004-07-22). Chimeric gene constructs for generation of fluorescent transgenic ornamental fish. ScholarBank@NUS Repository.
Abstract: Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
URI: http://scholarbank.nus.edu.sg/handle/10635/34928
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