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Authors: SZE, SIU KWAN 
Issue Date: 17-Nov-2004
Source: SZE, SIU KWAN (2004-11-17). DEVICE FOR ISOELECTRIC FOCUSSING. ScholarBank@NUS Repository.
Abstract: A device for separating one or more components in a sample is disclosed, the device including: (a) a first planar member (1) having a channel (2) along which a sample may be loaded and component(s) thereof focussed isoelectrically; and (b) means for exposing the channel (2) along at least a portion of its length and thereby exposing the sample or component(s) therewithin. The means for exposing the channel (2) may include a cover plate (7). A method of analysing a molecule is also provided, the method including: (a) providing an elongate open channel (2); (b) introducing a plurality of molecules into the elongate open channel (2); (c) separating molecules along the elongate open channel (2) according to their isoelectric points; (d) accessing a molecule in the elongate open channel (2) and analysing it. A use of the device in combination with a mass spectrometer, preferably a MALDI-TOF unit, for analysis of a protein is also disclosed.
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