Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34817
Title: METHODS OF ASSAYING DNA-REPAIRING ENZYMES AND THEIR ALKYLATED DERIVATIVES
Authors: LI, BENJAMIN FUK LOI 
AYI, TECK CHOON 
Issue Date: 30-Oct-1996
Source: LI, BENJAMIN FUK LOI,AYI, TECK CHOON (1996-10-30). METHODS OF ASSAYING DNA-REPAIRING ENZYMES AND THEIR ALKYLATED DERIVATIVES. ScholarBank@NUS Repository.
Abstract: The present invention provides methods of assaying the human DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) and/or active site alkylated derivatives thereof (R-MGMT), which rely on the ability of a protease, e.g. V8 Protease (also referred to as Glu-C Protease), to distinguish between these two enzyme forms. Such a method comprises the steps of: (a) contacting the sample with a protease to which one of human MGMT and R-MGMT is preferentially sensitive; and (b) determining whether the treated sample contains one or more polypeptides selected from (i) at least one polypeptide fragment characteristic of action of the protease on the protease-sensitive form of human MGMT and (ii) the protease-resistant form of human MGMT.
URI: http://scholarbank.nus.edu.sg/handle/10635/34817
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