Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34810
Title: Method of fixing biological tissue
Authors: KHOR, EUGENE 
TEOH, SWEE HIN 
CHIAN, KERM SIN
LOKE, WENG KEONG
Issue Date: 19-Jan-1994
Citation: KHOR, EUGENE,TEOH, SWEE HIN,CHIAN, KERM SIN,LOKE, WENG KEONG (1994-01-19). Method of fixing biological tissue. ScholarBank@NUS Repository.
Abstract: Biological tissue is treated with an aprotic solvent, e.g. dimethyl sulphoxide, to remove water from the tissue to render the tissue compatible for a reaction with a polyurethane or polyurethane precursor. The dehydrated tissue is reacted with (a) a solution derived from a diisocyanate to which a diol is subsequently added or with (b) a solution derived from a diisocyanate and a diol or with (c) an isocyanate terminated polyurethane solution or a combination of two or more of the solutions (a), (b), (c), using an aprotic solvent as reaction solvent under inert and anhydrous conditions at a temperature up to 60o Celsius. The treated tissue is recovered and rinsed with aprotic solvent to remove excess polyetherpolyurethane solution, disinfected and stored in a buffered saline solution.
URI: http://scholarbank.nus.edu.sg/handle/10635/34810
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