Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34732
Title: INDUCED PLURIPOTENT STEM CELLS FOR CARDIOVASCULAR DISEASE BASIC RESEARCH AND REGENERATIVE MEDICINE
Authors: WEI HEMING
Keywords: induced pluripotent stem cells, cardiomyocytes, mesenchymal stem cells, electrophysiology, hydrogen sulfide, cardiovascular diseases
Issue Date: 23-Sep-2011
Source: WEI HEMING (2011-09-23). INDUCED PLURIPOTENT STEM CELLS FOR CARDIOVASCULAR DISEASE BASIC RESEARCH AND REGENERATIVE MEDICINE. ScholarBank@NUS Repository.
Abstract: This study explored the potential of human induced pluripotent stem cells (hiPSCs) in the research and therapy of cardiovascular diseases. hiPSCs were derived from neonatal and adult dermal fibroblasts via retroviral transduction of OCT-4, SOX-2, c-MYC and KLF-4 and they were differentiated into cardiomyocytes (hiPSC-CMs) and mesenchymal stem cells (hiPSC-MSCs) via embryoid body formation. Electrophysiological and calcium imaging assays identified immature yet functionally adequate ventricular-, atrial-, and nodal-like hiPSC-CMs. H2S inhibited L-type Ca2+ current and outward rectifier potassium currents in hiPSC-CMs. Moreover, hiPSC-MSCs expressed typical surface markers and were capable of adipogenesis, chondrogenesis and osteogenesis. Compared with bone marrow MSCs, hiPSC-MSCs demonstrated superior self-renewal, pro-angiogenic and wound-healing potential. This study proves the feasibility of generating hiPSCs from young and older donors. It suggests that hiPSC-CMs are functionally adequate for in vitro modelling while highly self-renewable and functionally competent hiPSC-MSCs could serve as an alternative source to adult tissue-derived MSCs.
URI: http://scholarbank.nus.edu.sg/handle/10635/34732
Appears in Collections:Ph.D Theses (Open)

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1. Contracting EBs in suspension.mov19.48 MBVideo Quicktime

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2. A contracting EB attached on gelatin-coated surface.mov20.63 MBVideo Quicktime

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3. Dissociated single hiPSC-CM.mov17.44 MBVideo Quicktime

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4.Dissociated hiPSC-CMs with synchronized contraction.mov12.37 MBVideo Quicktime

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5. Dissociated hiPSC-CMs for calcium imaging.mov1.73 MBVideo Quicktime

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