Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34726
Title: ACCELERATED AND ENHANCED DIFFERENTIATION OF HUMAN NEURAL STEM CELLS BY A CELL-CONTACT MEDIATED MECHANISM
Authors: VIKNISH KRISHNAN KUTTY
Keywords: Neural Stem Cells, Neuronal Differentiation, Cell-Cell Contact, Co-culture, Gap Junctions, N-Cadherins
Issue Date: 25-Aug-2011
Source: VIKNISH KRISHNAN KUTTY (2011-08-25). ACCELERATED AND ENHANCED DIFFERENTIATION OF HUMAN NEURAL STEM CELLS BY A CELL-CONTACT MEDIATED MECHANISM. ScholarBank@NUS Repository.
Abstract: The standard culture methods tend to be inefficient and ineffective in the differentiation of neural stem cells (NSCs) into mature functional neurons. It was hypothesized that the reason could be because NSCs do not receive the necessary extrinsic cues for differentiation as they might in the in-vivo system. If these cues were provided in a favorable manner, neuronal differentiation could be made more efficient, and effective. In this thesis, I present a novel co-culture approach that uses a feeder layer consisting of predifferentiated NSCs on which GFP-tagged NSCs were seeded and co-cultured. Results show that NSCs co-cultured on the predifferentiated feeder layer undergo accelerated and enhanced neuronal differentiation as compared to cells grown under control conditions. This accelerated and enhanced neuronal differentiation is by a cell-contact mediated mechanism and experiments suggest a critical role for gap junctions and N-Cadherins.
URI: http://scholarbank.nus.edu.sg/handle/10635/34726
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