Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34684
Title: IDENTIFICATION OF THE RNA-BINDING PROTEIN DEAD END AS A LIMITING FACTOR FOR GERM CELL FORMATION
Authors: HONG NI
Keywords: Primordial germ cells, germline development, Oryzias latipes, dead end, limiting factor, cell culture
Issue Date: 30-Mar-2012
Source: HONG NI (2012-03-30). IDENTIFICATION OF THE RNA-BINDING PROTEIN DEAD END AS A LIMITING FACTOR FOR GERM CELL FORMATION. ScholarBank@NUS Repository.
Abstract: It is an ultimate goal to use structural information to infer function of macromolecules. My work was aimed at developing such systems in the model fish medaka (Oryzias latipes) utilizing the understanding of primordial germ cell (PGC) formation in early embryos, by identifying a limiting factor for PGC formation in vivo and in vitro. dead end (dnd) encodes an RNA-binding protein. We tested into its function by knockdown and gain-of-function techniques, and demonstrated its importance for PGC formation. Blastomeres from 64-cell embryos were found to be capable of single cell cultivation and PGC formation in culture, demonstrating that medaka PGCs are specified cell-autonomously. Lineage tracing in single cell culture revealed the presence of PGC precursors in medaka embryo at the 64-cell stage, which is increased upon dnd RNA injection. Altering dnd expression enables efficient germline chimera production and complete germline replacement, acting as a limiting factor controlling germ cell specification.
URI: http://scholarbank.nus.edu.sg/handle/10635/34684
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
HongNi.pdf3.42 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

170
checked on Jan 19, 2018

Download(s)

25
checked on Jan 19, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.