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Title: Formulation of Microbial Cocktails for Biodegradation of Btex
Keywords: BTEX, biodegradation, synthetic consortia, population dynamics, microbial interactions
Issue Date: 14-Nov-2011
Citation: KARTHIGA NAGARAJAN (2011-11-14). Formulation of Microbial Cocktails for Biodegradation of Btex. ScholarBank@NUS Repository.
Abstract: Pseudomonas putida F1 and Pseudomonas stutzeri OX1 were used to formulate synthetic consortia for BTEX biodegradation. Benzene and toluene supported the growth of both the strains; while ethyl benzene and o-xylene were utilized as growth substrates by P. putida F1 and P. stutzeri OX1, respectively. In a mixed substrate system, P. putida F1 exhibited incomplete removal of o-xylene while P. stutzeri OX1 displayed cometabolic removal of ethyl benzene with dark coloration of the growth medium. Biodegradation characteristics of synthetic consortia formulated using the two strains were evaluated for three model effluent systems. The co-culture enhanced the biodegradation of benzene and toluene through contribution from the two strains and allowed the individual bacterium to consume their respective growth substrates, ethyl benzene and o-xylene. Population dynamics studies revealed competition among the strains for benzene and toluene, and synergistic interaction to biodegrade ethyl benzene and o-xylene to completion.
Appears in Collections:Ph.D Theses (Open)

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